Page 10 - CANNABIDIOL (CBD) Critical Review Report Geneva, 4-7 June 2018 World Health Organization
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Figure 2: Biogenesis of THC and CBD adapted from Taura et al. (2007)
THCA synthase and CBDA synthase catalyze oxidative cyclization of the
monoterpene moiety of CBGA to form THCA and CBDA, respectively. THC
and CBD are generated from THCA and CBDA by non‐enzymatic
decarboxylation. [11]
In addition to genetic characteristics, cultivated plants are influenced by
environmental conditions and production technology during their life cycle. A
study evaluating the effects of ambient temperature and humidity, soil
temperature and precipitation on the content of THC and CBD in industrial
hemp noted that these agroclimatic conditions have differing effects on THC
and CBD. For example, CBD content is positively affected by soil temperature
and ambient temperature, but negatively influenced by precipitation [13]
E. Chemical Properties
Melting point: 62-63°C
Solubility: approx. 23.6 mg/mL in DSMO and ethanol [14]
F. Identification and Analysis
There are a number of published methods for the analytical detection of CBD
in various biological samples. For example,
▪ spectrophotometric determination [15];
▪ liquid chromatography–tandem mass spectrometry (LC–MS/MS)
detection of CBD in whole blood [16, 17] samples;
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